Isicatshulwa sembewu yomdiliya luhlobo lwe polyphenols ekhutshwa kwimbewu yomdiliya. Ubukhulu becala iqulunqwe procyanidins, catechins, epicatechins, gallic acid, epicatechin gallate kunye nezinye polyphenols.
uphawu
Umthamo we-Antioxidant
Imbewu yomdiliya isicatshulwa yinto yendalo ecocekileyo. Yenye yezona antioxidants zisebenzayo ezivela kwimithombo yezityalo. Uvavanyo lubonisa ukuba umphumo wayo we-antioxidant ngama-30 ~ 50 amaxesha e-vitamin C kunye ne-vitamin E.
umsebenzi
I-Procyanidins inomsebenzi onamandla kwaye inokuthintela i-carcinogens kwi-cigarettes. Ukukwazi kwabo ukubamba i-radicals yamahhala kwisigaba esinamanzi yi-2 ~ 7 amaxesha aphezulu ngaphezu kwe-antioxidants jikelele, njenge-α- Umsebenzi we-tocopherol ungaphezu kokuphindwe kabini ukuphakama.
isicatshulwa
Kwafunyaniswa ukuba phakathi kwezicubu ezininzi zezityalo, umxholo we-proanthocyanidins kwimbewu yamagilebhisi kunye ne-pine bark extract yayiyeyona iphakamileyo, kwaye iindlela eziphambili zokukhupha i-Proanthocyanidins kwimbewu yomdiliya yayiyi-solvent extraction, i-microwave extraction, i-ultrasonic extraction kunye ne-supercritical CO2 extraction. Imbewu yomdiliya isicatshulwa seproanthocyanidin iqulethe ukungcola okuninzi, efuna ukucocwa ngakumbi ukuphucula ubunyulu beproanthocyanidins. Iindlela zokucoca ezisetyenziswa ngokuqhelekileyo ziquka i-solvent extraction, i-membrane filtration kunye ne-chromatography.
Uxinzelelo lwe-Ethanol lube nelona futhe libalulekileyo kwisantya sokutsalwa kwembewu yomdiliya iproanthocyanidins, kwaye ixesha lokutsalwa kunye namaqondo obushushu akhange kubekho mpembelelo ibalulekileyo kumyinge wokutsalwa kwembewu yomdiliya iproanthocyanidins. Iiparameters zokutsalwa ezizezona zilandelayo: i-ethanol concentration ye-70%, ixesha lokukhupha i-120 min, umlinganiselo oqinileyo-ulwelo 1:20.
I-static adsorption experiment ibonisa ukuba izinga eliphezulu le-adsorption ye-hpd-700 ye-proanthocyanidins yi-82.85%, ilandelwa yi-da201, eyi-82.68%. Kukho umahluko omncinci. Ngaphezu koko, amandla e-adsorption yezi resin zimbini zeproanthocyanidins nazo ziyafana. Kuvavanyo lwe-desorption, i-da201 resin inezinga eliphezulu lokuchithwa kwe-procyanidins, eyi-60.58%, ngelixa i-hpd-700 ine-50.83% kuphela. Idibaniswe ne-adsorption kunye ne-desorption experiments, i-resin ye-da210 yayizimisele ukuba yi-resin ye-adsorption engcono kakhulu yokwahlula i-procyanidins.
Ngokusebenzisa inkqubo yokuphucula, xa i-concentration ye-proanthocyanidins yi-0.15mg / ml, izinga lokuhamba ngu-1ml / min, isisombululo se-ethanol se-70% sisetyenziswa njenge-eluent, izinga lokuhamba ngu-1ml / min, kwaye inani le-eluent yi-5bv, isicatshulwa. yembewu yomdiliya iproanthocyanidins inokucocwa kwangaphambili.