Grape noob extract yog ib hom polyphenols muab rho tawm los ntawm cov noob txiv hmab txiv ntoo. Nws yog tsim los ntawm procyanidins, catechins, epicatechins, gallic acid, epicatechin gallate thiab lwm yam polyphenols.
yam ntxwv
Antioxidant muaj peev xwm
Grape noob extract yog ib yam khoom ntshiab huv. Nws yog ib qho zoo tshaj plaws antioxidants los ntawm cov nroj tsuag. Qhov kev sim qhia tau hais tias nws cov nyhuv antioxidant yog 30 ~ 50 npaug ntawm cov vitamin C thiab vitamin E.
kev ua si
Procyanidins muaj zog ua haujlwm thiab tuaj yeem tiv thaiv cov carcinogens hauv cov luam yeeb. Lawv lub peev xwm los ntes cov dawb radicals nyob rau hauv aqueous theem yog 2 ~ 7 lub sij hawm siab dua li cov antioxidants dav dav, xws li α- Cov kev ua ntawm tocopherol yog ntau tshaj ob zaug siab.
rho tawm
Nws tau pom tias ntawm ntau cov ntaub so ntswg, cov ntsiab lus ntawm proanthocyanidins nyob rau hauv grape noob thiab ntoo thuv bark extract yog siab tshaj, thiab lub ntsiab txoj kev ntawm extracting Proanthocyanidins los ntawm grape noob yog hnyav extraction, microwave extraction, ultrasonic extraction thiab supercritical CO2 extraction. Grape noob proanthocyanidins extract muaj ntau impurities, uas yuav tsum tau ntxiv purification los txhim kho lub purity ntawm proanthocyanidins. Feem ntau siv purification txoj kev muaj xws li hnyav extraction, membrane filtration thiab chromatography.
Ethanol concentration muaj qhov cuam tshuam tseem ceeb tshaj plaws ntawm kev rho tawm ntawm cov noob txiv hmab txiv ntoo proanthocyanidins, thiab lub sijhawm rho tawm thiab qhov kub thiab txias tsis muaj qhov cuam tshuam tseem ceeb ntawm kev rho tawm ntawm cov noob txiv hmab txiv ntoo proanthocyanidins. Qhov zoo tshaj plaws extraction tsis tau raws li nram no: ethanol concentration 70%, extraction lub sij hawm 120 min, solid-kua piv 1:20.
Qhov kev sim adsorption zoo li qub qhia tau hais tias qhov siab tshaj plaws adsorption ntawm hpd-700 rau proanthocyanidins yog 82.85%, ua raws li da201, uas yog 82.68%. Muaj qhov txawv me ntsis. Ntxiv mus, lub peev xwm adsorption ntawm ob resins rau proanthocyanidins kuj yog tib yam. Hauv kev kuaj desorption, da201 resin muaj qhov siab tshaj plaws desorption ntawm procyanidins, uas yog 60.58%, thaum hpd-700 tsuas muaj 50.83%. Ua ke nrog adsorption thiab desorption thwmsim, da210 resin tau txiav txim siab los ua qhov zoo tshaj plaws adsorption resin rau kev sib cais ntawm procyanidins.
Los ntawm cov txheej txheem optimization, thaum lub concentration ntawm proanthocyanidins yog 0.15mg / ml, tus nqi txaus yog 1ml / min, 70% ethanol tov yog siv raws li eluent, tus nqi txaus yog 1ml / min, thiab tus nqi ntawm eluent yog 5bv, cov extract. ntawm grape noob proanthocyanidins yuav ua ntej purified.